Figure 5. STZ-induced diabetic mice have aberrant expression of niche related molecules.

(A) Percent changes in the expression of mRNA levels of Cxcl12 in FACS sorted nestinGFP⁺ cells before and after G-CSF treatment relative to control nestinGFP⁺ cells before G-CSF treatment and normalised to GAPDH (ΔΔCTmethod). Columns represent mean ± s.e.m., n=6 * p<0.05 and **p<0.01. (B) Representative FACS plots and graph bars showing percentages of CXCL12⁺ cells among nestin⁺ cells. Data are mean ± s.e.m., n=6, * p<0.05. (C) Concentration (pg/ml) of CXCL12 in BM extracellular fluid from control and STZ-treated mice. Data are mean number ± s.e.m., n=6. (D) Schematic representation of the experimental design used to selectively disrupt osteoblastic cells (see methods). (E) Donor-derived CD45.2⁺ cell engraftment 4 weeks after transplantation of mobilized peripheral blood in lethally irradiated SJL recipients. Columns represents mean± s.e.m., n=8 in each group, ^{◆◆◆, □□□, ★★★} p<0.001 ^{**, ●●, ▽▽, ##} p<0.01, ^■ p< 0.05