Skip to main content
. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Anal Chem. 2013 Jul 15;85(15):7271–7278. doi: 10.1021/ac401165s

Figure 1.

Figure 1

Image acquisition and analysis. (A) Schematic of the process flow for image acquisition, data analysis and cell identification. Imaging criteria for scanning the micropallet array is first entered into a ScanArray GUI that controls imaging by brightfield and fluorescence microscopy. Images are then processed with a combination of background subtraction, thresholding, size-based filtering and negative control filtering to identify target cells. Micropallets carrying specified cells are then selectively detached from the array and magnetically collected. (B) Photograph of large micropallet array. A U.S. quarter is shown next to the array for size comparison. (C–D) Micrographs of HeLa cells admixed with a low abundance of GFP-HeLa cells on micropallets. Brightfield and fluorescence images show identification of a single GFP-HeLa cell. Insets show the GFP-HeLa at higher magnification. The ‘star’ highlights array regions with water infiltration around the micropallets.