Table 1.

Parameters used for deriving the best fit of Eq. 3 to frequency-dependent elution time data for DS19 cells (Fig. 2) and for calculations of the mapping characteristics between elution profiles and cell biophysical characteristics as described in the text.

Parameter	Value	Units	Explanation
fstart	160 × 103	Hz	Sweep start frequency
fend	15 × 103	kHz	Sweep end frequency
Tsweep	600	s	Sweep time
R	7.5 × 10−6	m	Cell radius
g	9.81	m.s−2	Acceleration due to gravity
H	600 × 10−6	m	Channel height
W	25 × 10−3	m	Channel width
L	300 × 10−3	m	Channel length
V	1.42	V	Applied DEP volts p-p
η	1.002 × 10−3	N.s.m−2	Dynamic viscosity of water at 20°C
σs	30 × 10−3	S.m−1	Eluate conductivity
S	50 × 10−6	m	Electrode element width and spacing
F	4 and 10	mL.min−1	Flow rate
Rpol	0.375/(L × W)	Ω.Hz½	Frequency-dependent electrode polarization resistance
Cpol	11.3 × L × W	F. Hz½	Frequency-dependent electrode polarization capacitance
Rbulk	8s/(σs × L × W)	Ω	Bulk resistance of eluate on DEP electrode
Cbulk	690 × L × W/(8* s)	pF	Bulk capacitance of DEP electrode
ρp	1058*	Kg.m−3	DS19 cell density
f0	74*	kHz	DS19 cell crossover frequency
F (Mcyt, Mmem)	0.11*	dimensionless	DS19 HDLF geometric scaling function

^*Values derived from the best fit of the DEP-FFF theory.