Table 1.

depFFF tumor cell recovery was measured for tumor cells mixed into blood cell suspensions of different concentrations under 10%, 50% and 90% chamber loading conditions. Tumor cell recovery efficiency fell with increasing blood cell concentration and increasing chamber loading. The effect of changing the tumor: blood cell ratio was also measured with the blood cell concentration and chamber loading held constant. Tumor cell recovery efficiency did not depend on the tumor cell load at the tumor cell: blood cell ratios studied.

Blood cell concentration (l	Tumor: normal cell ratio	chamber length loading	Total cell load	Tumor cell load	Recovery of tumor cells
2.00E+05	1:1000	10%	1.00E+05	100	92%
2.00E+05	1:1000	50%	5.00E+05	500	70%
2.00E+05	1:1000	90%	9.00E+05	900	40%
1.00E+06	1:1000	10%	5.00E+05	500	52%
1.00E+06	1:1000	50%	2.50E+06	2500	41%
1.00E+06	1:1000	90%	4.50E+06	4500	25%
5.00E+06	1:1000	10%	2.50E+06	2500	15%
5.00E+06	1:1000	50%	1.25E+07	12500	12%
5.00E+06	1:1000	90%	2.25E+07	22500	10%
2.00E+06	1:1000	50%	5.00E+06	5000	49%
2.00E+06	1:400	50%	5.00E+06	2000	39%
2.00E+06	1:100	50%	5.00E+06	500	44%
2.00E+06	1:40	50%	5.00E+06	200	42%
2.00E+06	1:10	50%	5.00E+06	56	48%