Table 2. Statistical analysis of the conservation grade of the G-4 nef sequences or their G-4 patterns.

G-4 name	G-4 sequence or pattern	Conservation grade ( %)
		Group M	Clade A	Clade B	Clade C
Nef8528	G GAG GAG GTG G G	13.9%	0.6%	27.2%	0.3%
A)	G2 X1 G2 X1 G2 X1-2 G2	15.7%	2.4%	29.9%	0.5%
B)	G2 X0-7 G2 X0-7 G2 X0-7 G2	46.8%	57.6%	66.3%	9.3%
Nef8547	C CAGTCAGAC CTCAGGTAC CTTTAAGAC C	24.1%	0.0%	39.2%	11.1%
A)	C2 X7 C2 X7 C2 X7 C2	98.6%	97.6%	99.1%	98.5%
B)	C2 X1-10 C2 X1-10 C2 X1-10 C2	99.0%	99.4%	99.1%	98.9%
Nef8624	G GGGG GACTG GAAG G G	66.4%	13.3%	86.5%	58.8%
A)	G2 X2 G2 X3 G2 X2-3 G2	98.0%	95.2%	97.9%	98.7%
B)	G2 X0-7 G2 X0-7 G2 X0-7 G2	99.9%	99.4%	99.9%	100.0%

In bold bases possibly involved in G-4 folding.