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. 2013 Aug 27;8(8):e71512. doi: 10.1371/journal.pone.0071512

Figure 6. Biophysical characterization of the bEbs1-6 and Ebs1/bEbs1–6 complex.

Figure 6

(A) ITC calorimetric titrations. (▪): Titration of Ebs1 (35 μM) with bEbs1–6 (387 μM). (□): The bEbs1–6 (30 μM) binding specificity was tested by titration with NCS-wt (350 μM). (B) Size Exclusion Chromatography (Superdex 200 prep grade Hiload 16/60) of the selected bEbs1–6 and Ebs1 (?):SEC elution profile of bEbs1–6 alone; (∇): elution profile of Ebs1 alone; (?): elution profile of an equimolar mixture of Ebs1 (55 nmol) and the binder bEbs1–6 (55 nmol).