Figure 1. Enzyme-inhibitory complexes in normal pool plasma after activation with ellagic acid or dextran sulphate.

The levels of inhibitory complexes were measured by ELISA after activation of normal pool plasma with either ellagic acid or dextran sulphate (DXS; Mr 500 000). Normal pool plasma was incubated for 25 minutes with either ellagic acid (0.25 mg/ml) or DXS (0.25 mg/ml) at 37^oC in Hepes-buffer (25 mM Hepes, 150 mM NaCl, pH = 7.5). The reaction was stopped with stop buffer containing STI and polybrene. The volume of plasma was 50% of total volume during the activation with ellagic acid or DXS, and diluted further in the assay. A.U. = Arbitrary units.