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. 2013 Aug 27;8(8):e74043. doi: 10.1371/journal.pone.0074043

Figure 2. Enzyme-inhibitory complexes after activation with ellagic acid in samples from patients and healthy controls.

Figure 2

Levels of enzyme-inhibitory complexes were measured by ELISA in samples from patients with HAE-C1INH and healthy controls after activation with ellagic acid, without (black) and with (grey) addition of C1 esterase inhibitor (C1INH) after activation. Plasma was activated with ellagic acid (0.25 mg/ml) for 15 minutes, than C1INH or Hepes-buffer (25 mM Hepes, 150 mM NaCl, pH = 7.5) was added and after 10 minutes stop buffer containing STI and polybrene was added. The volume of plasma was 50% of total volume during the activation with ellagic acid, and diluted further in the assay. Significant differences are indicated in Table 3. The line represents the median value; A.U. = Arbitrary units.