Figure 4. The down-regulation of reporter activity by astakine 3′-UTR242–483 in Sf21 cells co-transfected with vector expressing astakine 3′-UTR as the competitor.
Luciferase activity assay was conducted in Sf21 cells which was co-transfected with plasmid pGL3-OpIE2-Ast 3′-UTR242∼483 and competitor plasmid pEGFP-CMV-Ast 3′-UTR242∼483. Luciferase activity is expressed relative to pGL3-OpIE2 vector without 3′-UTR and data represent mean ±S.D. (n = 6). Different letters represent statistically significant differences as compared to each plasmid-transfected Sf21 cell according to the Duncan's multiple range test (p<0.05).