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. 2013 Aug 27;8(8):e71579. doi: 10.1371/journal.pone.0071579

Figure 6. Iron-based quenching of Trp fluorescence of HbpS proteins.

Figure 6

(A–C) HbpS proteins were treated with increasing concentrations (up to 10 mM; marked by the arrow) of ferrous iron ions, as indicated in the Material and Methods section. The fluorescence spectra of the wild-type (WT) as well as of the mutants D141A and E78A/E81A were subsequently measured. (D) Differences in fluorescence (ΔF) were plotted against the concentrations of titrated ferrous iron for HbpS WT (▪), D141A (▴) and HbpS E78A/E81A (•).