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. 2013 Aug 27;8(8):e73261. doi: 10.1371/journal.pone.0073261

Figure 2. Generation of Malme-3M-luc cells.

Figure 2

(A) Schematic representation of the lentivirus vectors 3.7EF-1α-ER-△LNGFR-CMV-luciferase and 3.7CMV-ER-BirA. (B) Malme-3M cells were transduced with both 3.7lnluc and 3.7BirA lentiviral vectors and luc-positive cells were isolated by streptavidin beads. (C) FACS analysis of LNGFR expression in isolated Malme-3M-luc cells. Cells were labeled with anti-LNGFR monoclonal antibody CD271 followed by goat-anti-mouse-PE-CY5. Histogram indicates LNGFR expression, in which the shaded histogram represents purified Malme-3M-luc cells, and the black line represents un-transduced Malme-3M cells. (D) The bioluminescence image signal was measured with the Xenogen IVIS system, and serial dilution of Malme-3M-luc cells were plated in black 96-well plate in PBS containing D-luciferin substrate at final concentration of 0.15 mg/ml. (E) Pearson’s correlation coefficient analysis. The correlation coefficient (R) between the luciferase quantity and living cell number is 0.999.