Table 2. Primers for quantitative real-time RT-PCR (Rat).

Gene	Primer sequences	Anneal temperature (°C)	Product (bp)
GAPDH	F:5′GGAAAGCTGTGGCGTGAT3′ R:5′AAGGTGGAAGAATGGGAGTT3′	60	308
GPR30	F:5′CGCTCAAGGCAGTCATACCA3 ′ R:5′CCCCTGTCCGTTTTCCTCTA3′	60	96
ERβ	F:5′TGCTGGATGGAGGTGCTAATG3′ R:5′GAGGTCGGGAGCGAAAATG3′	60	81
ERα	F:5′GGAGACTCGCTACTGTGCTGTGT3′ R:5′AGTCATCTCTCTGACGCTTGTGC3′	60	281