Table 1. Composition of standard and experimental diets.
Standard diet | Control and experimental diets | ||||
CONTROL | LEU | WHEY | HIGH PROT | ||
Ingredients | |||||
Casein | 166 | 166 | 166 | - | 156 |
Whey | - | - | - | 144 | 144 |
L-cystine | 1.8 | 1.8 | 1.8 | - | - |
Alanine1 | - | 59 | - | - | |
Leucine | - | - | 44.5 | - | - |
Valine2 | - | - | 5.1 | - | - |
Isoleucine2 | - | - | 9.8 | - | - |
Proline | - | - | 5.7 | - | |
Choline | - | - | 2.5 | 2.5 | |
Rapeseed oil | 30 | 30 | 30 | 30 | 30 |
Peanut oil | 27 | 27 | 27 | 27 | 27 |
Sunflower oil | 3 | 3 | 3 | 3 | 3 |
Cellulose | 35 | 35 | 35 | 35 | 35 |
Sucrose | 100 | 100 | 100 | 100 | 100 |
Lactose | 134 | 134 | 134 | 134 | 134 |
Wheat flour | 458.2 | 399.2 | 398.8 | 482 | 334 |
Mineral mixture AIN93 | 35 | 35 | 35 | 35 | 35 |
Vitamin mixture AIN93 | 10 | 10 | 10 | 10 | 10 |
Given during | adaptation/immobilization | recovery | recovery | recovery | recovery |
Quantities are expressed in g.kg−1 dry matter for the CONTROL, LEU, WHEY and HIGH Prot groups.
LEU, leucine; WHEY, whey protein; HIGH PROT, high protein.
Alanine was included in the control diet to render the diets isonitrogenous. This amino acid has no effect on muscle protein metabolism.
Valine and isoleucine were included in the leucine-supplemented diet to prevent the fall of their plasma concentrations induced by leucine supplementation.