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. 2013 Aug 27;8(8):e72300. doi: 10.1371/journal.pone.0072300

Figure 5. ESRP1 is expressed in spermatogonial stem cells.

Figure 5

A. Spermatogonial stem cells (SSCs) were characterised by qRT-PCR analysis. RQ is relative quantity (n = 4). B. Western blot analysis shows that ESRP1 is expressed in ES cells, in GPSCs as well as in SSCs. ES cells and GPSCs were lysed after 30 minutes’ preplating to remove Mefs, while SSCs were scraped off together with the Mef-feeder layer for lysis. The primordial germ cell marker, MVH was enriched in SSCs versus the other cell lines. Actin was used for normalisation (n = 2). 3T3 fibroblasts were used as negative control. C. Confocal microcroscopy analysis revealed that ESRP1 is both nuclear and cytoplasmic in the SSCs as well. MVH staining was used as positive control. Scale bar is 5 µm.