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. 2013 Aug 27;8(8):e72300. doi: 10.1371/journal.pone.0072300

Figure 6. ESRP1 binds to pluripotency-related mRNAs.

Figure 6

A. RNA-immunoprecipitation (RIP) was performed using an anti-ESRP1 antibody. qRT-PCR was employed to analyse for the presence of Oct4, Nanog, Sox2, c-Myc and Lin28 mRNA bound by ESRP1. ESRP1-depleted samples were used as negative control; RQ is relative quantity (n = 3). 18s mRNA was equally immunoprecipitated in both cell types and average threshold cycle (Ct) is shown. B. 5′UTR reporter luciferase assay of Scr and ESRP1-depleted ES cells transfected with pGL3-pCMV-5′UTR-Oct4 and pGL3-pCMV-5′UTR-Sox2 versus Basic pGL3 vector; A.U. is arbitrary unit (n = 6).