Figure 4. PLK1 abrogation inhibits GBM tumor growth in-vivo.
(A) U251 cells were implanted subcutaneously into nude mice and tumor growth was monitored over time. Tumors grown to ≈172 mm3 were randomized to one of four treatment arms: vehicle treated control, GSK461364A (25 mg/kg), GSK461364A (50 mg/kg), or GSK461364A (100 mg/kg). Mice were treated with two doses of GSK461364A or DMSO control four days apart. Tumor volume plotted as the mean volume ± SD. (B) Cell lysates from control and GSK461364A treated tumors were measured for PLK1 protein levels by Western blot analysis using actin as a loading control. PLK1/actin ratio is shown.