(A) Pathology following HSV-1 infection. Corneas of C57BL6,
IRF-3−/− , IRF-7−/−
and DKO mice were scarified and inoculated with 2 × 106 pfu/eye. Disease
scores were recorded in accordance with parameters detailed in the materials and
methods. Data represents disease scores from three independent experiments (n
≥ 12 mice per group). (B) Survival of wild-type,
IRF-3−/−, IRF-7−/− and
DKO mice following corneal infection with 2 × 106 p.f.u HSV-1
strain 17 per eye. Survival curves were carried out independent of other studies
and represent data from three individual experiments. (n ≥ 12 mice per
group). Brain stems (C) and brains (D) were harvested at specified days
following infection of wild-type, IRF-3−/− ,
IRF-7−/− and DKO mice with 2 × 10 p.f.u.
HSV-1 strain 17 per eye. Graphs represent mean +/− SEM of two
independent experiments, (n ≥ 5 mice per group)