(A) In vitro replication in bone marrow derived dendritic cells. Primary
BMDCs were infected with HSV-1 strain 17 at MOI 1. At indicated times post
infection, cell supernatants were harvested and viral titers assayed on vero
cell monolayers. Results shown represent the mean +/− SEM of two
independent experiments, each carried out in triplicate. (B) Migratory ability
of primary BMDCs. The ability of wild-type, single and DKO BMDCs to migrate
towards the chemokine CCL19 was determined by counting the number of cells which
migrated through a 2 µm pore size polycarbonate filter of a transwell
plate. Results shown represent one experiment carried out in duplicate. The
experiment was repeated twice with identical results. Statistical analysis
(ANOVA) was performed using GraphPad Prism 5.0. (* , P ≤ 0.05; ** , P
≤ 0.01; ***, P ≤ 0.001).