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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Virology. 2013 Jun 16;444(0):71–79. doi: 10.1016/j.virol.2013.05.034

Figure 4. HSV replication in wild-type and IRF-3−/−IRF-7−/−and IRF3/7−/−(DKO) BMDCs and impact on DC migration.

Figure 4

(A) In vitro replication in bone marrow derived dendritic cells. Primary BMDCs were infected with HSV-1 strain 17 at MOI 1. At indicated times post infection, cell supernatants were harvested and viral titers assayed on vero cell monolayers. Results shown represent the mean +/− SEM of two independent experiments, each carried out in triplicate. (B) Migratory ability of primary BMDCs. The ability of wild-type, single and DKO BMDCs to migrate towards the chemokine CCL19 was determined by counting the number of cells which migrated through a 2 µm pore size polycarbonate filter of a transwell plate. Results shown represent one experiment carried out in duplicate. The experiment was repeated twice with identical results. Statistical analysis (ANOVA) was performed using GraphPad Prism 5.0. (* , P ≤ 0.05; ** , P ≤ 0.01; ***, P ≤ 0.001).