Figure 1. bleomycin treatment decreases the expression and phosphorylation of the Cav-2 beta isoform. (A) Immunoblot analyses of the expression of Cav-2 and its phosphorylated isoforms in lung lysates of wild-type mice treated with saline or bleomycin. β-actin is shown as a control for equal protein loading. Four mice are shown for each group (n = 7–10 for each group). (B) Densitometric analyses of the expression of Cav-2 and its phosphorylated isoforms were performed with the ImageJ software. The expression of Cav-2 and its phosphorylated isoforms were then normalized to β-actin expression and expressed as a percentage of the WT-SAL group. Bleomycin treatment decreases the protein levels of total Cav-2 β (***P = 0.0003) and PY19-Cav-2 β (****P < 0.0001) (n = 7–10 for each group).