Figure 1. (A)HEK293 cells stably transfected with the expression vector pcDNA3-c-mybHA or with the empty vector pcDNA3HA were tested for c-myb expression by quantitative reverse transcriptase real-time PCR (qRT-PCR). The expression level of c-myb in pcDNA3HA transfected cells was arbitrarily set to 100.(B)Western blot analysis of pcDNA3-c-mybHA- and pcDNA3HA-transfected cells. Anti-c-Myb and anti-HA antibodies were used for detection. β-actin expression was used to control equal loading. (C)qRT-PCR was performed for detection of each miRNA of the miR200 family. miR150 and miR155 were used as controls of non-regulated and c-Myb-regulated miRNA, respectively. The expression level of each miRNA in the pcDNA3HA-transfected cells was arbitrarily set to 100. Data are reported ± standard deviation. Statistical significance was calculated by two-tailed Student t test., ** P ≤ 0.01. qRT-PCR analyses were performed in triplicate in 2 independent experiments.