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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Virology. 2013 Jun 16;444(0):1–11. doi: 10.1016/j.virol.2013.05.036

Figure 2. Innate immune deficiencies and expression of miR122 facilitate replication of HCV replicons in mouse fibroblasts.

Figure 2

Immortalized MEFs (iMEFs) of the indicated genotypes were lentivirally transduced with GFP or miR122 and then transfected with replication-competent (wild-type) or defective (GNN) bicistronic subgenomic (JFH1) constructs expressing blasticidin S deaminase (bsd) gene that confers resistance blasticidin selection. Cells were selected in blasticidin-containing medium and stained with crystal violet after fixation.