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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Virology. 2013 Jun 16;444(0):1–11. doi: 10.1016/j.virol.2013.05.036

Figure 3. Persistent HCV RNA replication in mouse fibroblasts on immunodeficient backgrounds.

Figure 3

Immortalized MEFs of the indicated genotypes or Huh7.5 cells were transfected with full-length J6/JFH1 (FLR) or subgenomic JFH1 replicons expressing blasticidin S deaminase and cell populations selected in the presence of blasticidin. A. Quantification of HCV NS5A antigen positive cells over six passages following HCV RNA transfection. B. Representative flow cytometry plots of IFNαβR-/- and Huh7.5 cells stably harboring FLR- or SGR-stained for HCV NS5A antigen. C. Quantification of HCV RNA over several passages in Huh7.5 and STAT1-/- iMEFs stably replicating SGRs or FLRs as indicated. D. STAT1-/- iMEFs stably replicating SGRs or FLRs were grown in the presence or absence of 2′CMA (2mM) and stained with crystal violet after fixation. LOQ = limit of quantitation