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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Virology. 2013 Jun 16;444(0):1–11. doi: 10.1016/j.virol.2013.05.036

Figure 4. Stable HCV RNA replication in mouse fibroblasts expressing HCV entry factors and miR122 following infection with Jc1-5AB bsd.

Figure 4

A. Schematic of the experimental set-up. B. Crystal violet stained blasticidin-resistant colonies of the indicated cell populations infected with Jc1-5AB bsd or Jc1p7nsGluc2A. C. Immunohistochemical staining of STAT1-/- iMEFs expressing human HCV entry factors stably infected with Jc1-5AB bsd or non-infected control cells. D. Flow cytometry quantification of HCV NS5A antigen in STAT1-/- iMEFs expressing human HCV entry factors stably infected with Jc1-5AB bsd. E. Flow cytometric analysis of ectopic expression of human CD81, OCLN, CLDN1 and SCARB1 before (upper panels) and after transduction (middle panels) and after infection and selection (lower panels). F. Correlation between human CD81/OCLN and NS5A antigen positivity in Jc1-5AB bsd infected STAT1-/- iMEFs. G. miR122 expression in Jc1-5AB bsd infected or non-infected STAT1-/- iMEFs transduced with miR122 and HCV entry factors, untreated iMEFs and Huh7.5 cells.