Figure 6. Production of infectious HCV in mouse fibroblasts supplemented with apoE.
A. Schematic of the experimental set-up. B. Infection of naïve Huh7.5 cells with supernatants from cultures of STAT1-/- iMEFs transduced with mouse apoE, eGFP, mock transduced iMEFs, and Huh7.5. NS5A staining was performed 72 h post infection and the number of infectious virions quantified in a limiting dilution assay. LOD = limit of detection