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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Fluoresc. 2013 May 31;23(5):975–987. doi: 10.1007/s10895-013-1224-8

Figure 3.

Figure 3

A. The ability of the soluble fluorescent dye Rhodamine 110 (485ex/530em) to diffuse across an 8 μm pore polycarbonate membrane was determined by adding Rhodamine to the top of a Boyden chamber and at the indicated time point determining the level of fluorescent dye that was in the lower chamber.

B. Level of retained calcein-AM within Jurkat cells at 30 and 180 minutes after labeling.

C. Level of calcein retention by Jurkat cells after a 4 hour exposure to increasing concentrations of SDF-1.