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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Virology. 2013 Jul 24;444(0):363–373. doi: 10.1016/j.virol.2013.07.002

Figure 3. Depletion of B1 impairs intermediate transcription following WT virus infection, and can be rescued by BAF depletion.

Figure 3

(A) L-929 cells were transfected with 100 nM of siRNA siControl, siB1R-1, or siB1R-2. At 24 hours post transfection, cells were infected with WT virus at a MOI of 3 and total RNA harvested at 4hpi. Following reverse transcription, cDNA was quantified by qPCR. (B) L929 stably expressing shBAF or shControl were transfected with 100nM siRNA specific to B1 kinase (siB1-1) or siControl for 12h, and then transfected with pG8-Luciferase for 7hr, then infected at 37°C with WT virus at MOI=3 in the presence of 50 uM AraC. An infection of L929 with ts2 at MOI=3 in the presence of AraC at 37°C was also performed. Lysates were prepared at 12h after infection and assayed for luciferase activity, and RLU normalized to protein level. Data were obtained from three independent experiments performed in triplicate wells. Data from a representative experiment is shown. Error bars represent standard deviation. (*** indicates a p-value <0.05)