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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Virology. 2013 Jul 24;444(0):363–373. doi: 10.1016/j.virol.2013.07.002

Figure 5. The role of B1 kinase and BAF in viral intermediate gene expression is promoter dependent.

Figure 5

(A) L929 stably expressing shBAF or shControl were transfected with siRNA specific to B1 kinase (siB1-1) or siControl for 12h, and then transfected with 1 ng of T7-Luciferase for 7hr, then infected with 37°C with Wt-VTF7.3 at MOI=3 in the presence of AraC. Lysates were prepared at 12h after infection and assayed for luciferase activity, with RLU shown normalized to protein level. (B-C) L929 stably expressing shBAF or shControl were transfected with the indicated amounts of minP-Luc (B) or CMV-Luc (C). Lysates were prepared at 24h after transfection and assayed for luciferase activity, with RLU shown normalized to protein level. For all graphs, data were obtained from three independent experiments performed in triplicate wells. Data from a representative experiment is shown. Error bars represent standard deviation. (*** indicates a p-value <0.05)