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Nrf-2 is necessary for the life-extending effects of broccoli. Transcripts encoding Nrf-2 (a), Jnk-1 (b), Foxo-1 (c), Sirt-1 (d) and Sirt-3 (e) were knocked down in T. castaneum by RNAi, achieved by injecting dsRNA. Galleria mellonella IMPI was used as a control because there is no ortholog in T. castaneum. ***p < 0.001
