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Preparation procedure for Lip-Se liposomes. The DPPC, DSPG and Chol were dissolved in a chloroform and methanol mixture and allowed to form a thin film by rotoevaporation. The thin film was hydrated with distilled water containing 2 mg sodium selenite for 0.5 h to form multilamelllar vesicles followed by high-pressure homogenization. The Lip-Se suspensions were lyophilized using lactose monohydrate as a cryoprotectant
