Table II.

Data Analysis of Tryptic Digests from MonoPEGylated and DiPEGylated Amylin

Tryptic digest	Mna	Mwb	Pdc
Free mPEG-SC-NHS	5,435	5,481	1.008
Free mPEG-SPA-NHS	5,443	5,482	1.007
monoPEGylated (m/z 5,776 to 7,291)	6,435	6,448	1.002
diPEGylated (m/z 10,549 to 12,759)	11,613	11,643	1.002
Estimated mass of the conjugated mPEG	11,613 − 6,435 = 5,178	11,643 − 6,448 = 5,195
Estimated mass of the PEGylated peptide	6,435 − 5,178 = 1,257	6,448 − 5,195 = 1,253

Data obtained from the mass spectra as shown in Fig. 7

Mn = Σ(Ni * Mi)/ΣNi

Mw = Σ(Ni * Mi²)/Σ(Ni * Mi)

Pd = Mw/Mn

N _i = intensity at given m/z; M _i = major m/z peaks