Fig. 2.

Proteolytic activity of GhCP1 and AtCP2. a Sketch of GhCP1 and AtCP2 domains; b SDS-PAGE of purified proteins. His-tag fusion proteins of GFP, GhCP1 and AtCP2 were expressed in E. coli; c Proteolytic activity of purified His-GhCP1 and His-AtCP2. Purified proteins were added in reaction buffer and incubated at room temperature for indicated times as described in “Materials and methods”; d Effect of gut fluids on proteolytic activity of GhCP1 and AtCP2. Purified His-GhCP1 and His-AtCP2 proteins (final concentration: 2 mg/ml) were added to reaction buffer and incubated at room temperature for 1 h. +1 μl of gut fluid of 5th instar larvae was added to 100 μl reaction buffer; −no gut fluid added. Data are shown as mean ± SD