Fig. 6.

Effects of cotton overexpressing GhCP1 on bollworm larvae. a Expression of GhCP1 in cotton. Nine 35S:GhCP1 lines were analyzed by qRT-PCR, GhCP1 expression in wild-type cotton (R15) leaves was set to 1; Accumulation of gossypol in midgut and weight increase of tested larvae. 3rd instar larvae were fed on leaves of wild-type (R15) and 35S:GhCP1 (two independent lines: #3 and #4) cotton for 7 days. Gossypol accumulation in midgut (b) and bollworm weight increase (c) were recorded. * P < 0.05, ** P < 0.01; d The level of gossypol equivalents in tested cotton plant leaves; e Expression of DpCPV genes S1 (AY163247), S3 (AY167578) and S4 (AF542082) in bollworm midgut. 3rd instar larvae were fed with wild-type (R15) or 35S:GhCP1#3-1 cotton leaves for one day and then infected with DpCPV; after 2 days, total RNAs from midguts were extracted and subject to qRT-PCR. The expression of DpCPV genes in the larvae pre-fed with wild-type leaves was set to 1. qRT-PCR analysis was biologically repeated for at least three times, error bars represent SD