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. 2013 Aug 28;4:331. doi: 10.3389/fpls.2013.00331

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Copyright © 2013 Khandakar, Haraguchi, Yamaguchi and Kitamura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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2-D Gel electrophoretic separations of Hyoscyamus albus root-tip proteins extracted from cultures exposed to Fe-replete (A) and Fe-deficient (B) conditions. Twenty μg of proteins extracted from 100 mg of H. albus root tips cultured under Fe-replete (A) and Fe-deficient (B) conditions for 5 days were separated on 7 cm IPG strips (pH5-8 linear gradient) using isoelectric focusing (IEF) in the first dimension, followed by AnyKd® gels in the second dimension. Gels were stained with Flamingo fluorescent stain. The prodigy rank number was used as a spot number; the number shown in yellow color is identified as hyoscyamine 6β-hydroxylase (H6H), the others are shown in black color. Molecular mass (kDa) and pI are indicated on the left-hand and upper axes, respectively.