Table 2.
MS/MS-based cross-species identification and characterization of the protein spots that showed significant volume increase under iron-deficient conditions.
| Spot No. | Protein name | Species | Accession no (NCBI) | Theoretical mass (kDa)/pI | Observed mass (kDa)/pI | PS/NMP† | SC (%)‡ | Fold (−Fe/+Fe) | Subcellular localization |
|---|---|---|---|---|---|---|---|---|---|
| CARBOHYDRATE METABOLISM | |||||||||
| 58 | UDP-Glucose pyrophosphorylase | Solanum tuberosum | P19595 | 51.87/5.70 | 77.8/6.2 | 52/1a | 7b | 1.7 | Cytoplasm |
| 28 | Phosphoglycerate mutase | Solanum tuberosum | AAD24857.1 | 61.26/5.42 | 77.8/6.3 | 64/3 | 6 | 1.8 | Cytoplasm |
| 29 | Phosphoglycerate mutase | Solanum tuberosum | AAD24857.1 | 61.26/5.42 | 76.0/6.3 | 48/2 | 3 | 1.7 | Cytoplasm |
| 22 | Enolase | Solanum lycopersicum | NP_001234080.1 | 47.79/5.68 | 47.6/6.7 | 75/2 | 8 | 2.0 | Cytoplasm |
| 32 | Fructose bisphosphate aldolase like protein | Solanum tuberosum | ABB29926.1 | 38.53/8.32 | 45.4/7.9 | 53/2 | 11 | 1.7 | Cytoplasm |
| 41 | Fumarase | Solanum tuberosum | CAA62817.1 | 53.38/6.51 | 60.7/7.7 | 56/1a | 2 | 1.6 | Mitochondria |
| DEFENSE RESPONSE | |||||||||
| 7 | Pyridoxine biosynthesis protein isoform A | Lotus japonicus | AAZ67141.1 | 33.09/5.92 | 41.2/6.3 | 57/2 | 9 | 2.9 | Cytoplasm |
| 9 | Chaperonin 21 precursor | Solanum lycopersicum | NP_001234423.1 | 26.56/6.85 | 29.4/5.3 | 63/2 | 9 | 2.8 | Plastid |
| 1 | Peroxidase 27 precursor putative | Ricinus communis | XP_002527239.1 | 35.67/8.89 | 46.5/7.1 | 52/1a | 5 | 9.4 | Secreted |
| 62 | Peroxiredoxin | Ipomoea batatas | AAP42502.1 | 20.76/8.80 | 23.1/6.8 | 94/3 | 21 | 1.5 | Mitochondria |
| 61 | Glutathione peroxidase | Solanum lycopersicum | NP_001234567.1 | 18.84/6.58 | 20.9/6.8 | 89/3 | 21 | 1.5 | Secreted |
| 15 | Superoxide dismutase [Cu-Zn] | Nicotiana plumbaginifolia | P27082.2 | 15.23/5.47 | 16.5/6.5 | 101/2 | 20 | 2.1 | Cytoplasm |
| STRUCTURE / DEVELOPMENT | |||||||||
| 59 | Actin | Bos taurus | P62739 | 42.01/5.24 | 30.8/5.7 | 59/2 | 9b | 1.5 | Cytoplasm |
| 21 | Ubiquitin-conjugating enzyme E2 13 variant | Solanum tuberosum | ABA40444.1 | 16.56/6.20 | 17.3/6.8 | 79/3 | 29 | 2.1 | Cytoplasm |
| 53 | Actin depolymerizing factor 3 | Gossypium hirsutum | ABD66505.1 | 16.11/6.74 | 17.35/6.8 | 74/2 | 14 | 1.6 | Cytoplasm |
| 5 | Galactose oxidase/ kelch repeat-containing protein | Medicago truncatula | XP_003596164.1 | 35.49/5.43 | 35.6/5.3 | 50/2 | 8 | 3.3 | Cytoplasm |
| AMINO ACID/PROTEIN METABOLISM | |||||||||
| 17 | Cysteine protease 14 | Trifolium repens | AAP32193.1 | 39.66/5.63 | 37.4/6.3 | 49/1a | 5 | 2.0 | Vacuole |
| 60 | Aspartic Protease | Nicotiana tabacum | ABG37021.1 | 54.78/5.53 | 41.2/6.6 | 67/2 | 7 | 1.5 | Secreted |
| SECONDARY METABOLISM/OTHERS | |||||||||
| 18 | Caffeoyl-CoA-O- methyltransferase 6 | Nicotiana tabacum | Q42945.1 | 27.79/5.30 | 32.4/5.5 | 59/1a | 4 | 1.6 | Cytoplasm |
| 23 | Caffeoyl-CoA-O-methyltransferase 1 | Nicotiana tabacum | O24144 | 26.96/5.41 | 32.4/5.6 | 78/2 | 8b | 2.0 | Cytoplasm |
| 49 | Soluble inorganic pyrophsphatase | Solanum tuberosum | Q43187.1 | 24.26/5.59 | 34.0/5.7 | 58/1a | 8b | 1.6 | Cytoplasm |
| 24 | 6,7-Dimethyl-8-ribityllumazine synthase | Nicotiana tabacum | AAQ04061.1 | 24.42/8.18 (15.1/5.53)* | 16.7/6.6 | 55/1a | 17b | 2.0 | Plastid |
†
Protein score (PS) and number of matched peptide (NMP) were obtained from Mascot search.
‡
Percentage of sequence coverage (SC) of identified peptides related to the corresponding sequence in database.
a
Annotated MS/MS spectra are provided in Supplemental data.
b
Based on SwissProt full sequence identified by Mascot search.
*
Due to the presence of significantly long (86 aa) transit peptide in the precursor sequence, theoretical mass/pI of the predicted mature protein is also indicated in parenthesis.