Table 3.
MS/MS-based cross-species identification and characterization of the protein spots that showed significant volume decreased under iron-deficient conditions.
| Spot No. | Protein name | Species | Accession no (NCBI) | Theoretical mass (kDa)/pI | Observed mass (kDa)/pI | PS/NMP† | SC (%)‡ | Fold (+Fe/−Fe) | Subcellular localization |
|---|---|---|---|---|---|---|---|---|---|
| CARBOHYDRATE METABOLISM | |||||||||
| 40 | Fructose bisphosphate aldolase like protein | Solanum tuberosum | ABC01905.1 | 38.62/7.51 | 47.6/7.5 | 66/2 | 9 | 1.7 | Cytoplasm |
| 55 | NAD-dependent malate dehydrogenase | Nicotiana tabacum | CAB45387.1 | 43.31/8.03 | 41.2/6.0 | 70/2 | 7 | 1.6 | Mitochondria |
| DEFENSE RESPONSE | |||||||||
| 71 | Ascorbate peroxidase | Nicotiana tabacum | BAA12918.1 | 27.45/5.43 | 32.4/6.5 | 203/6 | 33 | 1.5 | Cytoplasm |
| 38 | Predicted cationic peroxidase | Vitis vinifera | XP_002268412.1 | 34.25/9.36 | 43.3/5.7 | 48/1a | 4 | 1.7 | Vacuole |
| STRUCTURE/DEVELOPMENT | |||||||||
| 47 | Annexin p34 | Solanum lycopersicum | NP_001234104.1 | 35.80/5.37 | 35.7/6.0 | 48/2 | 9 | 1.6 | Cytoplasm |
| AMINO ACID/PROTEIN METABOLISM | |||||||||
| 46 | Elongation factor Tu | Arabidopsis thaliana | Q9ZT91 | 49.41/6.25 | 57.8/6.8 | 217/5 | 17b | 1.6 | Mitochondria |
| 70 | S-adenosylmetionine synthase | Brassica rapa | Q5DNB1.1 | 43.16/5.8 | 48.2/6.3 | 50/2 | 7b | 1.5 | Cytoplasm |
| 69 | S-adenosylmetionine synthase 2 | Solanum tuberosum | Q38JH8 | 42.70/5.6 | 47.9/6.4 | 52/2 | 8b | 1.5 | Cytoplasm |
| 44 | Nitrite reductase | Nicotiana tabacum | BAD15364.1 | 65.46/6.67 | 77.3/7.1 | 57/2 | 5 | 1.6 | Mitochondria |
| SECONDARY METABOLISM/OTHERS | |||||||||
| 66 | Sinapyl alcohol dehydrogenase | Populus trichocarpa | XP_002322822.1 | 38.94/6.23 | 55.1/6.1 | 68/2 | 5 | 1.5 | Cytoplasm |
| 30 | Hyoscyamine 6β-hydroxylase | Atropa belladonna | AEM91979.1 | 39.22/5.44 | 37.4/6.4 | 64/4 | 16 | 1.8 | Cytoplasm |
| 52 | Acetoacetyl-CoA thiolase | Nicotiana tabacum | AAU95618.1 | 41.27/6.47 | 55.1/7.3 | 59/2 | 7 | 1.7 | Cytoplasm |
| ETC/ATP SYNTHESIS | |||||||||
| 27 | NADH dehydrogenase Fe-S protein 1 | Solanum tuberosum | Q43644 | 79.97/5.87 | 89.3/6.1 | 45/2 | 4b | 1.6 | Mitochondria |
| 39 | ATP Synthase subunit alpha | Celtis yunnanensis | ADL63175.1 | 55.05/6.23 | 66.8/7.3 | 61/2 | 8 | 1.7 | Mitochondria |
†
Protein score (PS) and number of matched peptide (NMP) were obtained from Mascot search.
‡
Percentage of sequence coverage (SC) of identified peptides related to the corresponding sequence in database.
a
Annotated MS/MS spectra are provided in Supplemental data.
b
Based on SwissProt full sequence identified by Mascot search.