A small-scale proteomic approach reveals a survival strategy, including a reduction in alkaloid biosynthesis, in Hyoscyamus albus roots subjected to iron deficiency

. 2013 Aug 28;4:331. doi: 10.3389/fpls.2013.00331

Comparative protein profile	−Fe/+Fe
No. of spots changed^*	48
(Increased in abundance)		(30)
(Decreased in abundance)		(18)
No. of spots identified	36
(Increased in abundance)		(22)
(Decreased in abundance)		(14)

Twenty μg of proteins extracted from 100 mg of H. albus root tips cultured under Fe-replete and Fe-deficient conditions for 5 days were separated on small gels (7 × 7 cm).

To analyze differential protein accumulation between the two conditions of Fe availability, a threshold value (ANOVA at p < 0.05) was set at ≥1.5-fold change after normalization using Prodigy SameSpots®.