Figure 3.

Using fluxomics and genome scale models to link genotype to metabolic phenotype. From an annotated genome sequence, a stoichiometric model of metabolism can be constructed. That model can be solved via either prediction of an optimal flux phenotype (FBA) or measurement of actual flux phenotype (¹³C-MFA). These results can help suggest modifications for altering the phenotype of the cell in a desired manner. In this way, a synthetic biologist can design new strains, build them using genetic modification methods, and test their phenotypes before designing new modifications in an iterative fashion.