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. 2013 Aug 23;33(4):e00059. doi: 10.1042/BSR20130076

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Cell extracts of C. glutamicum WT and C. glutamicum IMpgm were analysed by native PAGE followed by enzyme activity staining (A). Total Pgm activity in cell extracts of C. glutamicum WT (filled circles) and C. glutamicum IMpgm (open diamonds) at different concentrations (0.05–20 mM) of glc-1-P (B). Data represent mean values of three independent determinations from two independent cultivations and were fitted according to the Michaelis–Menten equation.