Figure 3. Purification of the main Pgm isoenzyme of C. glutamicum, SDS/PAGE analysis of each step of the purification procedure: ce (crude extract), aec (anion exchange chromatography step wise elution), hic (hydrophobic interaction chromatography), aeg (anion exchange chromatography with gradient), gf (gel filtration) and PAGE ruler prestained protein ladder (m, MBI Fermentas) containing proteins of indicated molecular mass.
The arrow points at the band, which was assigned to pgm.