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. 2013 Aug 23;33(4):e00059. doi: 10.1042/BSR20130076

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Glycogen was determined enzymatically as glucose liberated after amyloglucosidase treatment (two determinations per sample). Three independent cultivations were performed, data from one representative experiment are shown; the S.D. of the glycogen content was <10%. Phenotypes of C. glutamicum IMpgm (pXMJ19-pgm) (C) and C. glutamicum IMpgm (pXMJ19) (D) during cultivation in minimal medium with 1% (w/v) maltose. Shown are phase-contrast images; pictures were taken after 7 h of cultivation.