Fig. 3.
Assessment of the homogeneity of the purified ficin related to HPLC chromatogram peak (III) by (a) SDS–PAGE pattern using 15% gel. Lane 1, molecular-mass markers; lane 2, purified ficin; (b) gel filtration using Superdex 75 column; and (c) re-chromatography using SP-Sepharose column.