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. Author manuscript; available in PMC: 2013 Aug 28.

Published in final edited form as: Mol Immunol. 2010 Apr 3;47(9):1692–1700. doi: 10.1016/j.molimm.2010.03.005

Fig. 8 — Alterations in ICAM-1 isoform expression do not affect regulatory T cell function. In vitro suppression assays were performed as described in the Materials and Methods using wild type, Icam1^tm1Alb, Icam1^tm1Jcgr, or Icam1^tm1Bay CD4⁺CD25⁻ responder or CD4⁺CD25⁺ regulatory T cells. Cultures were stimulated with soluble anti-CD3 and anti-CD28 antibodies for 72 hours and were pulsed with ³H-thymidine for the last 18 hours. A, The results are shown as fold-increase over baseline controls for WT CD4⁺CD25⁻ cells alone (n=6), control CD4⁺CD25⁻ cells with wild type CD4⁺CD25⁺ cells (n=7), wild type CD4⁺CD25⁻ cells with Icam1^tm1Alb CD4⁺CD25⁺ cells (n=5), wild type CD4⁺CD25⁻ cells with Icam1^tm1Jcgr CD4⁺CD25⁺ cells (n=4), and wild type CD4⁺CD25⁻ cells with Icam1^tm1Bay CD4⁺CD25⁺ cells (n=4). B, wild type Treg cells are able to suppress proliferation of Icam1^tm1Jcgr and Icam1^tm1Bay CD4⁺CD25⁻ T cells. The results shown are fold-increase over baseline controls for wild type CD4⁺CD25⁻ cells alone (n=6), wild type CD4⁺CD25⁻ cells with wild type CD4⁺CD25⁺ cells (n=7), Icam1^tm1Jcgr CD4⁺CD25⁻ cells alone (n=3), Icam1^tm1Jcgr CD4⁺CD25⁻ cells with wild type CD4⁺CD25⁺ cells (n=4), Icam1^tm1Bay CD4⁺CD25⁻ cells alone (n=4), Icam1^tm1Bay CD4⁺CD25⁻ cells with wild type CD4⁺CD25⁺ cells (n=4). Statistical analysis was performed using Anova (*, p≤0.1 and ***, p≤0.001).