Table 2.

Analytical figures of merit of the fluorometric assay developed for analysis of AFM1.

Analytical parameters	Experimental findings
Linear range	1–50 pg/mL*, 6.25–25 pg/mL#
LOD	1 pg/mL
IC50	100 pg/mL*, 86 pg/mL#
R.S.D.	1.1%*, 0.5%#
Adj. R 2	0.987*, 0.991#
Assay sample volume	40 µL
Analysis time	180 min
Sample throughput	128 samples (in triplicate) in 180 min

*PBS; ^#CRM-BD282.