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Expression and purification of SRH from E. coli GJ1158. Expression and purification analysis SRH under different concentrations of sodium chloride on 15% SDS-PAGE stained with coomassie brilliant blue R-250. Lane 1: SRH induced with 500 mM sodium chloride; Lane 2: SRH induced with 300 mM sodium chloride; Lane 3: Protein marker (14.3, 20.1, 29, 43, 66 97.4 kDa); Lane 4: Ist wash; Lane 5: IInd wash; Lane 4, 5, and 6: purified protein eluates.
