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. 2013 Aug 13;2013:297305. doi: 10.1155/2013/297305

Figure 6.

Figure 6

Time course of turbidimetric fibrin clot and platelet-rich clot lysis assay. Fibrin clot or platelet-rich clots were incubated with HBS or with recombinant protein SRH or SAK (100 nM) in a solution containing 1.5 μM plasminogen. The decrease in A 405 with time was used to calculate the relative clot turbidity at different time point. The experiments were repeated for three times and data represent the means ± S.D. (a) Platelet-rich clot lysis assay: T 50% of SAK and T 90% of SRH were found 226.98 ± 4.62 min and 76.54 ± 4.74 min, respectively, at the final concentration of 100 nM. Control clots treated with HBS only showed that readings were stable throughout the incubation period. (b) Fibrin clot lysis assay: The T 50% of SAK (186.70 ± 2.76 min) and T 50% of SRH (191.46 ± 6.65 min) were not significantly different at the final concentration of 100 nM.