Figure 2. The fate of L-glutamate in N. meningitidis and its role in sensitivity to oxidative stress.
A Uptake systems of L-glutamate and its predicted intracellular fates in N. meningitidis. L-glutamate can be converted into glutathione by the action of GshA and GshB. The sensitivity to H2O2 of bacteria grown in medium salt (61.6 mM, MCDA-2, Panels B and D) or low sodium (21.6 mM, MCDA-1, Panels C and E) conditions. Bacteria were incubated with concentrations of H2O2 ranging from 0-0.8 mM for 20 min, and the number of surviving bacteria determined by plating to solid media. The strains are given in the key, and error bars show the S.D. of assays performed in triplicate on at least three occasions.