Skip to main content
. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Mol Microbiol. 2011 Jul 20;81(5):1330–1342. doi: 10.1111/j.1365-2958.2011.07766.x

Figure 4. Interaction of primary PMNs with E. coli, S. flexneri and N. meningitidis.

Figure 4

Bacterial suspensions were added to wells with (+) or without (−) adherent human PMNs in 24-well microtiter plates at an MOI of 2. The survival of bacteria was determined by lysing cells and plating the contents of the wells to solid media. Results are shown as the percent survival compared with the number of CFU in the inocula. Both E. coli and S. flexneri survived significantly less well than H44/76 in PMNs; for H44/76, approximately 65% of the bacterial population survived following association with PMNs. Results are the means of assays performed with PMNs from four different donors on different occasions; error bars show the SD. Significance of bacterial survival with or without PMNs (indicated) was determined by the Student’s t-test.