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. Author manuscript; available in PMC: 2014 Feb 2.
Published in final edited form as: Nat Commun. 2013;4:2261. doi: 10.1038/ncomms3261

Figure 5. AMA1-RON2 inhibitor blocks junction formation.

Figure 5

(a) Transmission electron microscopy of showing the different stages RBC invasion in the presence of 2 μM cytochalasin D, namely, attachment (1), re-orientation (2), junction formation (3) and rhoptry bulb secretion (4). R: rhoptry, M: micronemes, V: vacuoles; White arrow: junction. Scale bars represent 250 nm. (b) The percentage of merozoites that are attached to RBCs in the presence (white bars) or absence (black bars) of the AMA1-RON2 inhibitor NCGC00015280/NCGC00262650. (c) The percentage of apically oriented merozoites in the presence (white bars) or absence (black bars) of the inhibitor that form a junction and RBCs with vacuoles (indicative of rhoptry bulb secretion). Numbers within each bar represent the number of merozoite-attached RBCs in each category. Data was pooled from two independent experiments without inhibitor and one each with inhibitor NCGC00015280 and NCGC00262650. Scale bars represent 250 nm. (d) AMA1 secretion from micronemes is not affected. Merozoites released from schizonts in the absence (control) or presence of inhibitors NCGC00015280 (60 μM) and NCGC00181034 (60 μM) were analyzed using polyclonal antibodies to AMA1. Scale bars represent 1 μm.