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. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Free Radic Biol Med. 2011 Jul 4;51(8):1533–1542. doi: 10.1016/j.freeradbiomed.2011.06.028

Fig. 3. TR1 inhibition does not affect TNF-induced degradation of cytosolic IκB-α, nuclear translocation of p50 and p65 or DNA binding activity of NFκB.

Fig. 3

(A) Cytosolic IκB-α and (B) nuclear p50 and p65 were measured by western blot in curcumin- and CDNB-treated cells that were either unstimulated (−) or co-stimulated (+) with TNF for 1 hour. Blots were re-probed for β-actin to demonstrate equal protein loading. (C) DNA binding activity was assessed by EMSA of nuclear extracts from cells treated as in (A) and (B). The position of the major DNA-binding species, the p50/p65 heterodimer is indicated. Asterisks mark the positions of the supershifted bands obtained by incubation with antibodies against p50 and p65 (lanes labeled “anti-p50” and “anti-p65”). Each of the experiments in this figure was repeated at least 3 times with similar results.