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. Author manuscript; available in PMC: 2013 Aug 28.
Published in final edited form as: Free Radic Biol Med. 2011 Jul 4;51(8):1533–1542. doi: 10.1016/j.freeradbiomed.2011.06.028

Fig. 4. LPS-stimulated TNF production by RAW264.7 cells is inhibited at the nuclear level by TR1 inhibitors.

Fig. 4

(A) TNF production by RAW264.7 murine macrophages. Cells were incubated with vehicle (DMSO) or the indicated concentrations of CDNB or curcumin (curc) in the absence or presence of 100 ng/ml LPS for 24 hours. TNF released into the conditioned media was measured by ELISA. (B) Cytoplasmic activation and nuclear translocation of NFκB in LPS-stimulated RAW264.7 cells. Cells were incubated with increasing concentrations of curcumin (0, 10 and 50 μM) or CDNB (0, 10 and 30 μM) and either left unstimulated (−) or were stimulated (+) with 100 ng/ml LPS for 15 minutes. Cytoplasmic IκB-α and nuclear p50 and p65 were determined by western blot. Blots were re-probed for β-actin to show equal loading.